DSIP
L-tryptophyl-L-alanyl-glycyl-glycyl-L-aspartyl-L-alanyl-L-seryl-glycyl-L-glutamic acid
DSIP is a naturally occurring nonapeptide identified in 1977 from rabbit cerebral venous blood, comprising nine amino acids in the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. This linear, hydrophilic peptide maintains its endogenous structure without synthetic modifications, making it ideal for sleep regulation research, stress response modulation, and central nervous system function studies in controlled experimental settings.
Complete Dosing Guide
Quick Reference
Administration Method
Intravenous or intraperitoneal injection in research settings
Step-by-Step Dosing Protocol
Dosing Guidelines
Research protocols use species-specific dosing: 25 nmol/kg IV in rabbits for sleep studies (Schoenenberger et al., 1978), 60 µg/kg IP in rats for stress response research (Graf & Kastin, 1986), and 100 µg/kg IP in mice for analgesia studies (Nakamura et al., 1983). All doses are reconstituted in sterile saline at 1 mg/mL concentration. Doses above 500 µg/kg may cause mild agitation in rats, establishing an effective therapeutic window.
Different animals need different amounts: rabbits get 25 nmol/kg through IV, rats get 60 µg/kg into their belly, and mice get 100 µg/kg the same way. Mix it in clean saltwater first at 1 mg/mL. Too much (over 500 µg/kg) might make rats a bit restless, so there's a sweet spot for each study.
Mixing & Injection
DSIP is administered via intravenous (IV) or intraperitoneal (IP) injection in research settings, reconstituted in sterile saline at 1 mg/mL under aseptic conditions. IV administration provides rapid onset within 15-30 minutes, while IP allows for easier handling in small animal models. Injection sites should be rotated to prevent irritation, and timing should align with circadian rhythms for sleep studies. Store reconstituted solution at 2-8°C or -20°C in low-protein-binding vials.
DSIP is administered via intravenous (IV) or intraperitoneal (IP) injection in research settings, reconstituted in sterile saline at 1 mg/mL under aseptic conditions. IV administration provides rapid onset within 15-30 minutes, while IP allows for easier handling in small animal models. Injection sites should be rotated to prevent irritation, and timing should align with circadian rhythms for sleep studies. Store reconstituted solution at 2-8°C or -20°C in low-protein-binding vials.
Clinical Administration Guidelines
Injection Protocol
- •Subcutaneous injection or intranasal administration
- •Rotate injection sites or alternate nostrils for intranasal
- •30 minutes before bedtime for optimal sleep effects
- •Avoid large meals before bedtime administration
Monitoring Schedule
- •Monitor sleep quality and stress levels daily
- •Weekly sleep study assessments
- •Monthly stress hormone and sleep biomarker panels
- •STOP if excessive sedation or cognitive impairment
Dosage Calculator
Medical Disclaimer
DSIP is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Any use should occur only under medical supervision in approved research settings.